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  • Qiao Y Spitz M R Shen H et

    2020-07-08

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    Qiao, Y., Spitz, M.R., Guo, Z., et al., 2002b. Rapid assessment of repair of ultraviolet DNA damage with a modified host-cell reactivation assay using a luciferase reporter gene and correlation with polymorphisms of DNA repair BCI-121 in normal human  Meta Gene 21 (2019) 100583
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    Contents lists available at ScienceDirect
    EBioMedicine
    Alterations of gastric mucosal microbiota across different stomach microhabitats in a cohort of 276 patients with gastric cancer
    Xiaosun Liu a,1, Li Shao b,1, Xia Liu b, Feng Ji c, Ying Mei a, Yiwen Cheng b, Fengping Liu b, Chongxian Yan a, Lanjuan Li b, Zongxin Ling b,
    a Department of Gastrointestinal Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China
    b Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China c Department of Gastroenterology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China
    Article history:
    Keywords:
    Gastric cancer
    Gastric microbiota
    Helicobacter pylori
    Stomach microhabitat
    Tumor microenvironment 
    Background: As part of the tumor microenvironment, the gastric microbiota play vital roles in tumor initiation, progression and metastasis, but stomach microhabitats are not always uniform. We aimed to characterize differ-ences of gastric microbiota in stomach microhabitats associated with gastric cancer (GC) development. Methods: A cohort of 276 GC patients without preoperative chemotherapy was enrolled retrospectively, and 230 normal, 247 peritumoral and 229 tumoral tissues were obtained for gastric microbiota analysis targeting the 16S rRNA gene by MiSeq sequencing. The microbial diversity and composition, bacterial co-occurrence correlations and predictive functional profiles were compared across different microhabitats.
    Findings: GC-specific stomach microhabitats, not GC stages or types, determine the composition and diversity of the gastric microbiota. Most notably, bacterial richness was decreased in peritumoral and tumoral microhabitats, and the correlation network of abundant gastric bacteria was simplified in tumoral microhabitat. Helicobacter pylori (HP), Prevotella copri and Bacteroides uniformis were significantly decreased, whereas Prevotella melaninogenica, Streptococcus anginosus and Propionibacterium acnes were increased in tumoral microhabitat. Higher HP colonisation influenced the overall structure of the gastric microbiota in normal and peritumoral mi-crohabitats. PiCRUSt analysis revealed that genes associated with nucleotide transport and metabolism and amino acid transport and metabolism were significantly enriched in tumoral microbiota, while gastric acid secre-tion was significantly higher in HP positive group of the tumoral microbiota.
    Interpretation: Our present study provided new insights into the roles of gastric microbiota in different stomach microhabitats in gastric carcinogenesis, especially the pathogenesis of HP. Fund: National Natural Science Foundation of China.
    © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).
    1. Introduction
    Gastric cancer (GC) is the leading cause of cancer-related mortality worldwide. According to the latest cancer statistics in China, 679,000 newly BCI-121 diagnosed GC cases and 498,000 GC related deaths occurred in 2015, indicating that GC is the second most common cancer after lung cancer [1]. Although the overall incidence and mortality have steadily declined in the past several decades [2], 80–90% of GC patients are diag-nosed with advanced-stage disease, with a 5-year survival rate of b30%
    Corresponding author at: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China.
    E-mail address: [email protected] (Z. Ling).
    1 These authors contribute equally to this work.