br APPBP contains a tetratricopeptide repeat
APPBP2 contains a tetratricopeptide repeat (TPR) structural motif that frequently mediates protein-protein interactions [28,29], so the binding of APPBP2 with PPM1D is potentially mediated by the TPR structural motif. Since APPBP2 (PAT1) phosphorylates Mei2 and Ste11 in the controls of sex differentiation  and functions as a positive reg-ulator of Kinesin motility, resulting in cargo transport , the
This work was supported by the Key Program of Natural Science Re-search of Higher Education of Anhui Province (grant no. KJ2017A241), the National Natural Science Foundation of China (grant no. 81772493) and the Science and Technology Program of Anhui Province (grant nos. 2017070503B037 and YDZX20183400002554). The funders had no role in the study design, data collection, data analysis, interpre-tation, or writing of the paper.
Declarations of interest
All co-authors agree with the content of this manuscript and report no conflicts of interest related to this study.
formed the experimental procedures: All authors; Analysed the data:
Fig. 6. PPM1D and SPOP attenuated the inhibition of cell migration and invasiveness induced by APPBP2 knockdown in NSCLC cells. (a) Effects of PPM1D and SPOP on cell migration and invasion of A549 stable 3-Indoleacetic acid by transwell, n = 3. (b) Effects of PPM1D and SPOP on cell migration and invasion of H1299 stable cells by transwell, n = 3. (c) The statistical results of PPM1D and SPOP on cell migration and invasiveness of A549 stable cells by transwell. A one-way ANOVA revealed a significant effect of group (F[6,14] = 35.7, p b 0.001). (d) The statistical results of PPM1D and SPOP on cell migration and invasiveness of H1299 stable cells by transwell. A one-way ANOVA revealed a significant effect of group (F[6,14] = 72.58, p b 0.001). The addition or non-addition of plasmid or virus is present as “+” and “-”, respectively. Data are presented as mean ± SEM. The difference between means was compared by Tukey's Multiple Comparison test. 0.001 b **p b 0.01 and ***p b 0.001 for indicated comparison. n.s. refers as not significant. Scale bars: 50 μm.
Appendix A. Supplementary data
Supplementary data to this article can be found online at https://doi.
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